ABSTRACT
Objective To exPlore the ProtectiVe mechanism of Dendrobium nobile lindl ( DNL ) decoction on the exPression of Peroxisome Proliferator actiVated recePtor gamma ( PPARγ) in the renal cortex of diabetic nePhroPathy ( DN) rats. Methods Sixty SD rats were randomly diVided into six grouPs (n=10 Per grouP) as follows: normal control grouP (NC), diabetic nePhroPathy control grouP ( DN) ,rosiglitazone grouP ( RGZ) ,the Dendrobium nobile lindl low ( LD) ,medium ( MD) and high ( HD) dose grouPs. After DN rat model was established, the rats were administrated with resPectiVe medications for 12 weeks,resPectiVely. The renal Pathology of rats was obserVed. The mRNA and Protein exPression of PPARγ in the renal cortex were detected via Real_time PCR and Western blotting,resPectiVely. Results High dose DNL decoction significantly alleViated thickening of kidney tissue basement membrane and fusion of foot Process in model rats. The leVels of PPARγmRNA and Protein exPression in the MD and HD grouPs were significantly increased as comPared with the DN grouP (P<0. 05,P<0. 01). Conclusion DNL decoction can effectiVely reduce kidney injury by uP_regulating the PPARγ mRNA and Protein exPression in diabetic nePhroPathy rats.
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of Cox7a2 on the LH-induced testosterone production and the involved autophagy regulating signals in TM3 mouse Leydig cells.</p><p><b>METHODS</b>The Cox7a2-pEYFP-N1 fluorescent protein vector was constructed and transfected into TM3 mouse Leydig cells. The level of testosterone was determined by ELISA, and the effects of Cox7a2 on the expression of the steroidogenic acute regulatory protein (StAR) and the phosphorylation of the autophagy regulatory factor P70S6K were detected by Western blot.</p><p><b>RESULTS</b>LH stimulation increased the StAR protein expression and testosterone production, while Cox7a2 decreased P70S6K phosphorylation, reduced StAR expression and consequently inhibited LH-induced testosterone biosynthesis in the TM3 Leydig cells.</p><p><b>CONCLUSION</b>Cox7a2 inhibits testosterone production by decreasing the StAR protein expression, which might be at least in part related with the activation of autophagy in TM3 mouse Leydig cells.</p>